This is one of a few different formulas i use in my shrimp tanks. This formula is used for the promotion of health, growth, strength, color & Breeding. Ingredients Bacillus Subtillis Natto 3.0x109 (3 billion cfu/g) Instantized BCAA Powder. (2:1:1 ratio of L-leucine. L-Isoleucine & L-Valine). Chitosan. (98% pure.) Product details #1. Bacillus subtilis, is a Gram-positive, catalase positive bacterium. #2. (BCAA) Branched-chain amino acid is an amino acid having aliphatic side-chains with a branch (a carbon atom bound to more than two other carbon atoms). Among the proteinogenic amino acids, there are three BCAAs: leucine, isoleucine and valine. #3. Chitosan is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). It is made by treating shrimp and other crustacean shells with the alkali sodium hydroxide. Info I mix the following amounts of all three products into a 2ltr glass bottle, and then add RO water. Give it a good shake for a few minutes and then leave it in a dark cool place for 14 days, only taking it out to give it a shake once a day. 0.5 Grams BSN + 2 Grams BCAA + 1.5 Grams Chito. Now I am no scientist and cannot explain in great detail how or why it works, but what I can say is that the shrimp that get this treatment of 5ml once a week added directly to the water, show exceptional colour, health and increased breeding rates. Also shrimp seem to breed and berry a little earlier than normal.